Please use this identifier to cite or link to this item:
https://ptsldigital.ukm.my/jspui/handle/123456789/485667| Title: | Imiquimod-loaded fish oil bigels and its effectiveness on treatment of non-melanoma skin cancer |
| Authors: | Khurram Rehman (P68017) |
| Supervisor: | Mohd Hanif Zulfakar, Dr. |
| Keywords: | Imiquimod Immune response modifier Chemotherapeutic agent Skin cancer Fish oil bigels Dissertations, Academic -- Malaysia |
| Issue Date: | 19-Feb-2016 |
| Description: | Imiquimod is an immune response modifier and serves as a chemotherapeutic agent for many skin associated diseases such as actinic keratosis, skin cancer etc. However, imiquimod is also associated with severe inflammatory side-effects that hamper its clinical potential. The aim of this project was to develop a new drug delivery vehicle system for imiquimod which will not only able to reduce the side-effects of imiquimod but also able to be therapeutically effective against skin cancer. To achieve this purpose, imiquimod was incorporated into fish oil and delivered via a "bigel" drug delivery system. Bigel is a colloidal mixture of hydrogel and oleogel and was developed and characterized to co-administer fish oil and imiquimod. Fish oil oleogel was chosen as the lipophilic portion of the bigel formulations due to its anti-inflammatory properties which would be able to reduce imiquimod side-effects. Different polymers were investigated for the hydrogel portion of the bigel formulations among which carbopol-fish oil bigel formulations exhibited the best vehicle features and were further investigated against animal and human skin cancer models. Molecular modeling and proton nuclear magnetic resonance (NMR) studies revealed a possible lipophilic complexation between fish oil and imiquimod which could help either the permeation of imiquimod across the skin or reducing the inflammatory side-effects of imiquimod. In vivo studies, including histopathology and immunological analysis, on Swiss Albino mice revealed added benefits of fish oil co-administration with imiquimod, which not only reduced the erythema and transepidermal water loss (TEWL) but were also found to be effective against induced tumor. Bigel formulations were also effective in significantly (P<0.05 one-way ANOVA) modulating immune response parameters such as vasculoendothelial growth factor (VEGF), tissue necrosis factor (TNFα), interferon gamma (INFγ) and interleukin (IL) 6 and 10 in mice. Fish oil omega-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) also exhibited its significant (P<0.05 one-way ANOVA) influence in human squamous and basal carcinoma cell lines growth inhibition and modulating immune response. Thus it can be concluded that the carbopol-fish oil bigel system can be utilized for topical delivery of imiquimod and omega-3 fatty acids of fish oil significantly reduced imiquimod-related side effects and effectively inhibited skin tumor growth,Ijazah Doktor Falsafah |
| Pages: | 256 |
| Call Number: | WR500.K45i 2016 9 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Pharmacy / Fakulti Farmasi |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_97249+Source01+Source010.PDF Restricted Access | 448.58 kB | Adobe PDF |  View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.