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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Ahmad Shuhud Irfani Zakaria, Dr. | |
| dc.contributor.author | Fayyadhah Binti Mohd Azmi (P87451) | |
| dc.date.accessioned | 2023-10-10T08:28:43Z | - |
| dc.date.available | 2023-10-10T08:28:43Z | - |
| dc.date.issued | 2019-05-31 | |
| dc.identifier.other | ukmvital:119497 | |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/485654 | - |
| dc.description | Uncaria gambir extracts consist of catechin, which has been shown to have anti-inflammatory and antibacterial properties. Traditionally, Uncaria gambir has been used for treatment of toothache and spongy gum, highlighting its potential use in dentistry. However, the cytotoxicity of this extract has never been investigated. The study aimed to determine the cytotoxicity of Uncaria gambir extracts towards fibroblast cells in vitro. Uncaria gambir extracts was dissolved in 1% DMSO and was diluted into two-fold dilutions with culture media into eight different concentrations, ranging from 7.8 μg/ml to 1000 μg/ml. After an initial incubation of 24 hours in the 96-well plates, MC3T3 fibroblast cells were treated with various concentrations of the extracts and incubated for 24 hours in triplicates. 10% DMSO and culture media were used as positive and negative controls respectively. Cell viability was measured using Alamar Blue, DNA Fluorescence and Neutral Red Uptake (NRU) assays. Morphological changes of the cell were determined using Field Emission Scanning Electron Microscope (FESEM) and LIVE/DEAD cell analysis. Both DNA Fluorescence and Alamar Blue assays showed no cytotoxic effects of the extract towards the fibroblast cells at all concentrations with the mean percentage of cell viability of more than 70%. However, NRU assay showed that the extract was not cytotoxic at concentration lower than 500 μg/ml and was cytotoxic at 1000 μg/ml. The half maximal inhibitory concentration (IC50) of the extract measured using Alamar Blue, DNA Fluorescence and NRU assays were 1751 μg/ml, 3109 μg/ml and 1031 μg/ml, respectively. Significant morphological changes of the cells were observed at 1000 μg/ml with marked shrinkage in size and ruptured cell membrane using FESEM. LIVE/DEAD cell analysis showed a mixture of red and green fluorescence in a low density cell population as compared to negative control. Conclusively, the Uncaria gambir extracts was not cytotoxic towards MC3T3 fibroblast cells at a concentration below than 500 μg/ml when tested in-vitro.,Ijazah Sarjana Doktor Pergigian Klinikal (Pergigian Pediatrik) | |
| dc.language.iso | eng | |
| dc.publisher | UKM, Kuala Lumpur | |
| dc.relation | Faculty of Pharmacy / Fakulti Farmasi | |
| dc.rights | UKM | |
| dc.subject | Fibroblast Growth Factors | |
| dc.subject | Universiti Kebangsaan Malaysia -- Dissertations | |
| dc.subject | Dissertations, Academic -- Malaysia | |
| dc.title | Cytotoxicity evaluation of uncaria gambir extract towards fibroblast cell | |
| dc.type | Theses | |
| dc.format.pages | 31 | |
| dc.identifier.callno | 9 Tesis Cd QU107. F286c 2019 | |
| Appears in Collections: | Faculty of Pharmacy / Fakulti Farmasi | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_119497+Source01+Source010.PDF Restricted Access | 1.7 MB | Adobe PDF |  View/Open |
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