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https://ptsldigital.ukm.my/jspui/handle/123456789/475569| Title: | Human respiratory epithelial cells derived from the nasal turbinate express mesenchymal stem cell markers |
| Authors: | Aisha A. Muhammed (P50309) |
| Supervisor: | Ruszymah Bt. Hj. Idrus, Professor Dr. |
| Keywords: | Human respiratory Epithelial cells Nasal turbinate express Mesenchymal stem cell Epitherial cell |
| Issue Date: | 2013 |
| Description: | The respiratory epithelium is the epithelium which lines majority of the conducting portion of the respiratory system. It provides an efficient barrier against microorganisms and harmful diseases. Since it is the first line of defence mechanism, respiratory epithelium can be damaged and need to be replaced to avoid healing by fibrosis which might results in tracheal stenosis. Tracheal tissue engineering is an alternative promising treatment modality which can reduce morbidity and mortality which occurred as a result of fibrosis. Mesenchymal stem cell markers are surface proteins which is responsible for some of these cells unique properties. Surface antigens CD73, CD90 and CD105 are important mesenchymal stem cell markers which used to characterize them. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping techniques. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping method: flow cytometry and immunocytochemistry. Respiratory epithelial cells were harvested from human nasal turbinate (n=6) and cultured using co-culture technique. Fibroblasts were removed at confluence leaving respiratory epithelial cells which were passaged further to passage four. Cells were evaluated for mesenchymal stem cell markers namely CD73, CD90, CD105 and the hematopoietic stem cell marker CD45 at passage 1 (P1) and passage 4 (P4). Flowcytometry and immunocytochemistry techniques were used to compare the level of expression of these markers. The results showed that human nasal respiratory epithelial cells expressed the mesenchymal stem cell markers at P1 and maintain the expression of these markers at P4. Data was analyzed using “student-t” test, Pvalue= 0.05. Using flowcytometry, comparing the values at P1 to P4 (mean� SEM) ; CD73=72.5�4.2% and 64.7�6.0% , P-value=0.3, CD90=69.2�7.4% and 72.0�1.7%, P-value=0.7, CD105=33.7�3.1% and 37.1�4.9%, P-value=0.5 , CD45= 7.3�3.9% and 13.3�1.5%, P-value=0.1. Using immunocytochemistry, comparing the values at P1 to P4; (mean� SEM) CD73=54.8�4.4% and 56.7�7.5%, P-value=0.8, CD90=40.0�4.4% and 34.1�2.9%, P-value=0.2, CD105=26.8�1.4% and 23.5�1.3%, P-value=0.1, CD45=1.0�0.0% and 6.5�1.8%, P-value=0.01. Values were not significant when compared between the two passages except for CD45. Both methods of immunophenotyping gave comparable results. This preliminary study indicates that human respiratory epithelial cells from the nasal turbinate expressed mesenchymal stem cell markers and maintained the stem cell phenotype till passage 4. That indicates the respiratory epithelial cells derived from nasal turbinate retain some of mesenchymal stem cells properties, and keep them even after serial passages. Therefore the nasal turbinate could be used as a source of respiratory epithelial cells for the respiratory mucosa tissue engineering.,Master/Sarjana |
| Pages: | 71 |
| Call Number: | tesis QS532.5.E7 .M952h 2013 9HUKM |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Medicine / Fakulti Perubatan |
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