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https://ptsldigital.ukm.my/jspui/handle/123456789/475558| Title: | Effects of piper sarmentosum on bone fracture healing in a postmenopausal osteoporotic rat model |
| Authors: | Mohamed R Abdalla Estai (P45926) |
| Supervisor: | Farihah Suhaimi, Professor Dr. |
| Keywords: | Piper sarmentosum Bone fracture Postmenopausal osteoporotic Rat model |
| Issue Date: | 2-Aug-2011 |
| Description: | Osteoporotic fractures are the clinical endpoint of osteoporosis. Previous studies have reported that osteoporosis is associated with a delay in fracture healing but clinical evidence is still lacking. Various factors such as transforming growth factor (TGF-β) and oxidative stress may be considered as pathogenic factors for bone loss and delayed fracture healing through inhibition of differentiation and induction of apoptosis of osteoblasts. Piper sarmentosum (P.s) is a herb commonly used in Malaysia as traditional medicine. Its extract has been reported to possess antioxidant, anticarcinogenic and anti-inflammatory properties. It also contains a natural scavenging antioxidant, naringenin. The aim of this study was to determine the effects of aqueous extract of P.s on fracture healing in oestrogen deficient osteoporotic rats by observing histological, radiological, biochemical and biomechanical changes. A pilot study was performed prior to the main study to determine the time needed for ovariectomy to induce osteoporosis based on bone structural histomorphometry. Forty eight female Sprague-Dawley rats weighing 200-250gm were randomly divided into 4 groups: (i) Sham-operated (SO) group; (ii) Ovariectomised-control (OVXC) group; (iii) Ovariectomised + oestrogen replacement therapy (OVX+ERT) (100 μg/kg /day) group; (iv) Ovariectomised + P.s extract (OVX+P.s) (125 mg/kg) group. The right femora of all the rats were fractured at the mid-diaphysis and K-wires were inserted for internal fixation six weeks after ovariectomy. Following the fracture, all the rats received the above treatment for another 6 weeks. The animals were then sacrificed and the fractured right femora were harvested. For the pilot study, bone volume/tissue volume (BV/TV), trabecular number (Tb.N) reduced significantly while trabecular separation (Tb.Sp) increased significantly in ovariectomised rats, compared to the control group 6 weeks post-ovariectomy (P<0.05). In the main study, the median histological fracture healing scores (Allen’s score) for the SO, OVX+ERT and OVXC+P.s groups were significantly higher, compared to the OVXC group. Under radiological examination, the median fracture healing scores (Warden’s score) for the SO, OVX+ERT and OVXC+P.s groups were significantly higher, compared to the OVXC group, while the median callus score and the mean axial callus volume for the SO, OVX+ERT and OVXC+P.s groups were significantly lower, compared to the OVXC group. Under biomechanical examination, the mean flexure load, flexure stress and Young’s modulus for the OVXC group were significantly decreased, compared to the SO, OVX+ERT and OVXC+P.s groups. Percentage change of the plasma level of TGF-β1 before treatment was decreased significantly in the OVXC, OVX+ERT and OVXC+P.s groups, compared to the SO group, while after 6 weeks of treatment, the level of TGF-β1 in the OVX+ERT group was increased significantly, compared to the OVXC and OVXC+P.s groups (P<0.05). Treatment with P.s extract showed improvement in biomechanical strength of the callus. Under radiological examination there was reduction in callus volume and callus score. There was evidence of improvement of fracture healing score as observed by histological examination. Flavonoid compound present in P.s through its free radical-scavenging activity, may be used effectively to improve healing of osteoporotic fractures. In conclusion, effective supplementation of P.s extract in the oestrogen-deficient state is beneficial for the fracture healing process.,Master/Sarjana |
| Pages: | 151 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Medicine / Fakulti Perubatan |
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