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Title:	Metabolite profiling of Epinephelus fuscoguttatus (Forsskål 1775) in response to Vibrio vulnificus infection
Authors:	Yosmetha Mayalvanan (P88228)
Supervisor:	Syarul Nataqain Baharum, Assoc. Prof. Dr.
Keywords:	Metabolites Epinephelus guttatus -- Southeast Asia Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	3-Jun-2019
Description:	Epinephelus fuscoguttatus is economically crucial to various Southeast Asia countries where they are reared in fish farms to meet the demand for supply. Vibriosis is a systemic infectious disease that has been steadily affecting the fish farming industry extensively these past few years and is caused by Vibrio spp., a pathogenic Gram negative bacterium. It has led to an increased rate of mortality among farmed fishes and a continuous economic loss in the commercial aquaculture industry. This study focuses on understanding the host's immune response and the pathways involved against vibriosis by profiling the metabolites in the groupers. Identification of significant metabolites that activates the immune responses of E. fuscoguttatus exposed to vibriosis and the consequent mapping of the metabolic pathways involved in the immune response to vibriosis. Mapping of the pathways are crucial to explaining the roles the metabolites play in fish immunity. A solvent extraction method was used on the grouper's immune organs (gills, liver and spleen) prior to LC-qTOF-MS and LC-qTOF-MS/MS analysis. Raw data generated from the analyses was pre-processed using Profile Analysis 2.0, normalised using MetaboAnalyst 3.0 which was also to conduct some statistical analyses. The data was then analysed using SIMCA P+ where the PCA score plot for this data set was found to be 88%. Compound identification was done using SmartFormula Bruker and METLIN databases and cross-referenced with MassBank and Metfrag databases. The metabolites found in this study were mostly amino acids (primary metabolites) and their corresponding abundances are as follows. Glutamine (0.44%), alanine (0.68%), phenylalanine (2.63%) and tyrosine (2.60%) were detected in high intensities in survived-infected gills. Aspartic acid (13.57%) and leucine (4.01%) were highly abundant in survived-infected livers and lysine was detected in high intensities in both gills (2.94%) and liver (3.64%) of the survived-infected fish. This research highlights the functions of amino acids in various immune related pathways that are activated in the E.fuscoguttatus in response to vibriosis. The findings of this research can be incorporated into fish feed as the amino acids act as supplements to strengthen fish immunity. Enhancing and strengthening fish immunity plays a big role in reducing the mortality of farmed groupers and in turn this contributes in securing the rate of grouper export.,Master of Science
Pages:	116
Call Number:	QP171.Y637 2019 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Institute of Systems Biology / Institut Biologi Sistem (INBIOSIS)

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