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Title:	Metabolomics analysis of mangosteen (Garcinia mangostana L.) seed development and germination
Authors:	Othman Mazlan (P88610)
Supervisor:	Wan Mohd Aizat Wan Kamaruddin, Dr.
Keywords:	Mangosteen -- Metabolism Seeds -- Development Germination Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	29-Jul-2019
Description:	Mangosteen (Garcinia mangostana L.) is a tropical fruit valued for its delectable taste and medicinal properties such as anticancer, antibacterial, antifungal and antioxidant. However, it produces recalcitrant seeds which are sensitive to desiccation and cold temperature. As such, the seeds cannot be stored for prolonged period and this prevents its mass propagation for commercial agriculture. G. mangostana seeds are also known to have active metabolism upon shedding, allowing for rapid energization during germination. Nonetheless, there is a lack of information regarding metabolite profiles for G. mangostana seed development and germination. Therefore, metabolomics approach via liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) was employed to profile seed development (six, eight, 10, 12, and 14 weeks after anthesis) and germination (zero, one, three, five, seven, and nine days after sowing). During development, G.mangostana seed sugar levels (glucose and fructose) increased continually throughout the period while the levels of secondary metabolites (flavonoid and organic acid) were increased from middle to late development. The elevated sugar levels may indicate seed priority in accumulating metabolites that can provide rapid energy production for germination. Meanwhile, the increase of secondary metabolites implies increasing seed protection during development in terms of structural integrity of seed coat and antipathogenic characteristics. During germination, sugar levels were decreasing, as they were probably being consumed by glycolysis to energize cellular growth and metabolism. Meanwhile, secondary metabolites such as alkaloids, flavonoids and xanthone-related metabolites exhibited pattern of transient increase. These metabolites may be responsible in seed defense against herbivory and pathogen infection, as well as alleviating cellular oxidative stress during cell wall modification and protrusion of radicle and plumule. In brief, this metabolomics study has shed light on important metabolites involved in G. mangostana seed development and germination. G.mangostana seed recalcitrance is dependent on the accumulation of certain metabolites such as citrate, glucose, galactose, glycerol, malate, and threonate compared to orthodox seed.,Master of Science
Pages:	107
Call Number:	QK495.G87O866 2019 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Institute of Systems Biology / Institut Biologi Sistem (INBIOSIS)

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