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Title:	Characterisation of bacterial aggregates as potential agent in heavy metal bioremediation
Authors:	Pranesha Prabhakaran (P80261)
Supervisor:	Wan Syaidatul Aqma Wan Mohd. Noor, Dr.
Keywords:	Soils -- Heavy metal content Soil pollution -- Measurement Soil remediation Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	13-Aug-2018
Description:	Heavy metal contamination is one of the environmental issue that could be consider as global threat as heavy metal could infiltrate deep into the bed of groundwater sources and surface water which end up in food chain. Bioremediation of heavy metal by using bacterial aggregates could enhance bioremediation process, at the same time offers a safer approach. Aggregates formation by microbes that occurs under unfavourable condition able to result in enhanced efficiency in bioremediation. Thus, this study focuses on exploiting bacterial aggregation ability to be applied in heavy metal bioremediation. The potential bacterial isolates for this research were isolated from a heavy metal contaminated site at Bestari Jaya, Selangor followed by aggregation, hydrophobocity and extra polymeric substances (EPS) analysis to analyse the process of bacterial aggregation under heavy metal stress. The viable CFU/mL for all the sampling site range from 0.7 × 102 to 3.4 × 104. Based on the heavy metal analysis of the water sample, tin, aluminum, zinc and manganese were the main heavy metal that present. The isolated bacteria were screened on minimal medium agar containing the heavy metals in order to choose the right isolate for the study. The isolates that showed growth on the minimal medium agar were used in heavy metal toxicity test. Based on the study, two isolates labeled as S22 and S6G showed potential in exhibiting growth until 4000 ppm of manganese and zinc. These isolates were identified based on API Identification kit and 16s rRNA sequencing as Burkholderia cepacia and Pseudomonas aeroginosa. Overall, the aggregation study on both isolates and heavy metals showed fluctuated pattern. Aggregation index (AI) and Bacterial Adhesion Toward Hydrocarbon (BATH) percentages were higher under zinc-stressed condition. As extrapolymeric substances play a major role in aggregation, biochemical analysis of extracted EPS were carried out to analyse the constituent. The analysis showed that main component of the EPS is protein which is significantly higher than carbohydrate. Overall, this study has showed the relationship between formation of bacterial aggregates, hydrophobicity, EPS formation and detoxification efficiency for heavy metal. Hence, it will be a good potential for application in bioremediation of toxic heavy metals in environment.,“Certification of Master's/Doctoral Thesis” is not available,Master of Science
Pages:	122
Call Number:	TD879.H4P733 2018 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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