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https://ptsldigital.ukm.my/jspui/handle/123456789/463728Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Aminah Abdullah, Prof. Dr. | - |
| dc.contributor.author | Shalini Markandan (P78259) | - |
| dc.date.accessioned | 2023-09-25T09:35:54Z | - |
| dc.date.available | 2023-09-25T09:35:54Z | - |
| dc.date.issued | 2018-08-07 | - |
| dc.identifier.other | ukmvital:103681 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/463728 | - |
| dc.description | In Malaysia, Bougainvillea glabra (bunga kertas) is cultivated as flowering ornamental plant for its colourful flower-like bract. The purpose of this study was to determine the effect of extraction solvents' polarities on phytoconstituent, antioxidant activity and safety in B. glabra bract. Preliminary qualitative phytochemical screening confirmed the presence of flavonoids, tannins, steroid and triterpenes. The B. glabra bract was extracted with several solvents (acetone, ethanol, and methanol) at different concentrations (50%, 70% and 100%) and 100% distilled water. B. glabra bract was tested for quantitative hytoconstituent analysis namely total phenolic, flavonoid and betalain content. The extract of 100% ethanol exhibited high phenolic and flavonoid content of 76.74 GAE mg/100 g and 250.10 QE mg/100 g respectively, followed 100% methanol extract exhibited phenolic and flavonoid content of 63.92 GAE mg/100 g and 249.5 QE mg/100 g respectively. The total betalain content in 100% methanol and 100% ethanol extract of B. glabra bract was found to content betacyanins 7.87 mg/kg and 6.58 mg/kg respectively whereas betaxanthins 5.98 mg/kg and 5.73 mg/kg respectively. B. glabra bract extracts were evaluated for antioxidant properties via seven antioxidant methods of different free radical species and mechanism of actions namely DPPH free radical scavenging activity, superoxide radical scavenging (XOD), ABTS radical scavenging, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), oxygen radical absorbance capacity (ORAC) and intracellular antioxidant activity in red blood cell (IAA-RBC). The 100% methanol and 100% ethanol of B. glabra bract extracts showed high antioxidant activity (IC50 < 1 mg/mL) in DPPH, XOD and ABTS assay systems. Both extracts also possessed good antioxidant reducing power FRAP and CUPRAC assay. The same extracts also showed good ORAC value of 167,946 µmol TE /100 g and 119,654 µmol TE /100 g respectively. The extracts have potential bioavailability which ethanol 100% (1.69%) has higher ability to penetrate RBC membrane than methanol 100% (1.01%). Significant positive correlations (P < 0.05) of antioxidant activity with TFC and TPC, whereas negative correlations in TBC were observed. The 100% ethanol extract was subjected to further analysis as this solvent is efficient in polyphenol extraction and safer than methanol. The HPLC-PDA analysis showed the presence of quercetin as primary flavonoid constituent, which conferred antioxidant activity. Further testing on cytotoxicity against WRL-68 human liver and monkey kidney vero cell lines showed IC50 value > 100 ug/mL. B. glabra bract was found to be free from harmful heavy metal (Pb, Hg, Cd and As) contamination. Microbial load analysis showed TAMC value is below permissible level whereas TYMC value is exceeded the limit. Thus, natural antioxidant substances in B. glabra bract scavenge excess free radicals and prevent oxidative damage by keeping the oxidative stress state in balance. In conclusion, the local B. glabra bract can serve as a new source of antioxidant in food and nutraceutical product development.,“Certification of Master's/Doctorla Thesis” is not available,Master of Science | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Bangi | - |
| dc.relation | Faculty of Science and Technology / Fakulti Sains dan Teknologi | - |
| dc.rights | UKM | - |
| dc.subject | Solvents | - |
| dc.title | The antioxidant activity, phytochemical and toxicity evaluation of Bougainvillea glabra bract using different type of solvents | - |
| dc.type | theses | - |
| dc.format.pages | 155 | - |
| dc.identifier.callno | QD544.M385 2018 tesis | - |
| dc.identifier.barcode | 003629(2019) | - |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_103681+SOURCE1+SOURCE1.0.PDF Restricted Access | 6.6 MB | Adobe PDF |  View/Open |
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