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Title:	Biobutanol production from agro-industrial wastes as substrates using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)
Authors:	Al-Shorgani Najeeb Kaid Nasser. (P48965)
Supervisor:	Wan Mohtar Wan Yusoff, Prof. Dr. Hj.
Keywords:	Waste products as fuel. Agro-industrial wastes Biobutanol production
Issue Date:	20-Oct-2011
Description:	The aim of this study is to produce biobutanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) from different agro-industrial wastes. The process parameters for acetone-butanol-ethanol (ABE) production using palm oil mill effluent (POME) by C. saccharoperbutylacetonicum N1-4 were investigated. Various pretreatment procedures including acidic, alkaline, enzymatic and combination between acidic and enzymatic methods were applied on POME, rice bran (RB) and de-oiled rice bran (DRB) in order to produce reducing sugars. This study also investigated the effect of butyric acid addition to the culture of C. saccharoperbutylacetonicum N1-4 on biobutanol production. The results show that the best conditions for ABE and biobutanol production from POME were; sedimented POME, 15% inoculum size, 5.8 pH, 30°C incubation temperature, zero agitation speed, and in the absence of crude palm oil. Among the pretreatment methods, enzymatic hydrolysis was the most suitable for ABE production from POME which produced the highest ABE productivity of 0.06 g/L.h and 0.043 g/L.h of biobutanol. Further treatment of POME hydrolysate with XAD-4 resin improved ABE production to 4.29 g/L with a yield of 0.29 g/g. For biobutanol, the production improved to 3.09 g/L. Dilute acid pretreatment for RB and DRB produced comparable reducing sugars to enzymatic hydrolysis. Pretreatment of 10% RB using dilute acid followed by enzyme resulted in total ABE production of 8.88 g/L with a yield of 0.28 g/g and a productivity of 0.07 g/L.h whereas, for 10% DRB the results were, 10.51 g/L, 0.40 g/g and 0.088 g/L.h, respectively. When RB and DRB hydrolysates were further treated with XAD-4 resin, the production of ABE increased to 9.92 and 12.13 g/L, respectively, and ABE yields also increased to 0.35 and 0.44 g/g respectively. Direct fermentation of 7% sago starch produced 16.65 g/L ABE in which 9.83 g/L is biobutanol with an ABE productivity of 0.15 g/L.h when supplemented with P2 medium while, only 13.33 g/L ABE was produced when supplemented with TYA medium with an ABE productivity of 0.11 g/L.h. The use of sago starch resulted in the highest concentrations of ABE and biobutanol. The study showed that the addition of 4 g/L butyric acid resulted in the best concentration of ABE (23.51 g/L) containing 17.71 g/L biobutanol with an ABE productivity of 0.2 g/L.h when TYA medium was used. C. saccharoperbutylacetonicum N1-4 was also detected to produce biobutanol in a limited nutrient medium containing glucose and butyric acid. Combination of 10 g/L of butyric acid and 20 g/L of glucose was found to produce 13 g/L of biobutanol which indicates the importance of glucose-butyric acid ratio for the enhancement of biobutanol production. As a conclusion, agro-industrial wastes such as POME, sago starch, RB and DRB have potential value to be used as feedstock for renewable energy production such as biobutanol.,Master
Pages:	201
Call Number:	TP360 .S476 2011
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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