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https://ptsldigital.ukm.my/jspui/handle/123456789/463411| Title: | Pemencilan dan pencirian sebatian kimia daripada buah Orania sylvicola (Arecaceae) |
| Authors: | Ummu Solihah Wan Ahamad Sukari (P54350) |
| Supervisor: | Prof Emeritus Dato' Dr. Ikram M. Said |
| Keywords: | Orania sylvicola Ekstrak heksana Ekstrak metanol Palms-analysis |
| Issue Date: | 26-Dec-2013 |
| Description: | Kajian telah dijalankan ke atas buah Orania sylvicola daripada famili Arecaceae (Palmae). Kajian ini penting kerana tiada kajian yang pernah dilakukan ke atas spesies Orania sylvicola. Ia merangkumi analisis fiziko-kimia minyak dan pemencilan sebatian kimia. Pengekstrakan ke atas buah O. sylvicola dibahagikan kepada dua ekstrak, iaitu ekstrak heksana dan ekstrak metanol. Ekstrak kasar heksana yang terdiri daripada minyak O. sylvicola dianalisis sifat fiziko-kimia seperti ujian kelikatan, indeks bias, takat lebur gelinciran, takat keruh, nilai iodin, nilai peroksida, nilai penyabunan, bahan bukan penyabunan, lengkuk peleburan dan penghabluran minyak serta komposisi asid lemak. Analisis kromatografi gas menunjukkan asid laurik (58.23%) merupakan asid lemak dominan dan kandungan keseluruhan asid lemak tepu dalam minyak lemak O. sylvicola ini mencapai sehingga 97%. Penyaringan fitokimia ke atas ekstrak kasar metanol pula menunjukkan ujian positif terhadap alkaloid, flavonoid dan juga steroid. Ekstrak kasar metanol difraksikan kepada kloroform dan n-butanol melalui kaedah pemfraksian. Pemencilan komponen kimia telah dilakukan dengan menggunakan kaedah kromatografi turus dan kromatografi radial. Bagi fraksi kloroform, satu campuran sterol iaitu stigmasterol dan β--sitosterol serta 5-hidroksi- 7,4'-dimetoksiflavon berjaya dipisahkan. Fraksi n-butanol pula memberikan asid 4- hidroksibenzoik dan 9H-Pirido[3,4-b]indol, juga dikenali sebagai β-karbolin. Kesemua sebatian ini dicirikan dengan pelbagai kaedah spektroskopi seperti Spektroskopi Resonans Magnetik Nukleus 1D dan 2D, Spektroskopi Jisim, Spektroskopi Infra Merah dan Spektroskopi Ultra Lembayung serta disahkan melalui perbandingan dengan kajian terdahulu. Kehadiran sebatian indol merupakan sebatian yang pertama kali dilaporkan dalam famili Arecaceae.,Studies were conducted on the fruits of Orania sylvicola from family Arecaceae (Palmae). This is important because there is no study have been done for this spesies. The study focused on the physicochemical analysis of oils as well as isolation of the chemical components. Extraction of the fruits O. sylvicola was divided into two parts, hexane and methanol extract. The hexane extract which consist of oils was analysed for their physicochemical properties including viscosity test, refractive index, iodine value, peroxide value, melting profiles and fatty acids compositions. Gas chromatography analysis showed that lauric acid (58.23%) appeared as the dominant fatty acid and the total content of saturated fatty acids present in O. sylvicola is up to 97%. Phytochemical screening in the methanol extract showed positive results for alkaloid, flavonoid and steroid. Methanol extract was then fractionated with chloroform and n-butanol through solvent partition. Isolation of the chemical components was performed by using column chromatography and radial chromatography. From the chloroform fraction, a mixture of sterols, known as stigmasterol and β--sitosterol and 5-hydroxy-7,4'-dimetoxyflavone were isolated and identified. The n-butanol fraction consisted of 4-hydroxybenzoic acid and 9HPirido[3,4-b]indole, also known as β-carboline. All of these compounds were characterized by various spectroscopic techniques such as Nuclear Magnetic Resonance Spectroscopy 1D and 2D, Mass Spectroscopy, Infrared Spectroscopy and Ultra Violet Spectroscopy and were confirmed by comparison with previous studies. The isolated indole alkaloid is the first time reported in the family, Arecaceae.,Master |
| Pages: | 123 |
| Call Number: | QK495.P17 U944 2013 |
| Publisher: | UKM, Bangi |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi |
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| ukmvital_81497+SOURCE1+SOURCE1.0.PDF Restricted Access | 3.53 MB | Adobe PDF |  View/Open |
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