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https://ptsldigital.ukm.my/jspui/handle/123456789/463409Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Prof. Dr. Shahrul Hisham Zainal Ariffin | |
| dc.contributor.author | Nurfathiha Abu Kasim (P59507) | |
| dc.date.accessioned | 2023-09-25T09:24:31Z | - |
| dc.date.available | 2023-09-25T09:24:31Z | - |
| dc.date.issued | 2014-06-05 | |
| dc.identifier.other | ukmvital:81486 | |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/463409 | - |
| dc.description | Rawatan ortodontik menyebabkan perubahan ke atas aktiviti penanda biologi yang melibatkan proses inflamasi (laktat dehidrogenase; LDH), nekrosis (aspartat aminotransferase; AST), penyerapan tulang (asid fosfatase rintang tartrat; TRAP) dan pembentukan tulang (alkali fosfatase; ALP). Objektif kajian ini adalah untuk menentukan profil penanda biologi semasa rawatan ortodontik serta hubung kaitnya dengan jumlah penanda biologi yang hadir. Sampel cecair krevis gingiva (GCF) diambil daripada 19 orang subjek ortodontik sebelum dan semasa rawatan ortodontik selama 5 minggu. Semua subjek menggunakan braket swa-peligaturan (self-ligating bracket; SLB) diberikan daya 1.0 N atau 1.5 N sama ada pada arkus maksila sebelah kanan atau kiri yang ditentukan secara rawak dengan kaedah lambungan syiling. Aktiviti spesifik enzim berkaitan dan analisis ELISA terapit bagi penentuan jumlah enzim yang hadir. Jarak pergerakan kanin (gigi taring) pula diukur dengan menggunakan pengukur digital. Analisa statistik dilakukan dengan menggunakan perisian SPSS ver. 20. Hasil analisis ujian t berpasangan dalam membandingkan aktiviti enzim basal dan semasa rawatan menunjukkan peningkatan aktiviti spesifik LDH yang signifikan (p<0.05) dalam GCF pada minggu 1, 2 dan 3 (mesial; daya 1.0 N) dan pada minggu 1, 2, 3 dan 4 (distal; daya 1.0 N), manakala pada minggu 1 hingga 5 (mesial dan distal; daya 1.5 N). Aktiviti spesifik AST menunjukkan peningkatan yang signifikan (p<0.05) pada minggu ke-4 dan 5 (mesial dan distal; daya 1.0 N) serta minggu 3, 4 dan 5 (mesial; daya 1.5 N) dan minggu 4 dan 5 (distal; daya 1.5 N). Aktiviti spesifik TRAP pula meningkat dengan signifikan pada minggu ke-5 (mesial; daya 1.0 N) dan pada minggu ke 3, 4 dan 5 (distal; daya 1.0 N; p<0.05), manakala dengan daya 1.5 N tiada peningkatan yang signifikan pada kedua-dua bahagian (mesial dan distal; p>0.05). Sementara itu, aktiviti spesifik ALP tidak menunjukkan sebarang peningkatan yang signifikan (p>0.05) pada mesial dan distal (1.0 N dan 1.5 N). Selain itu, perbandingan di antara daya 1.0 N dan 1.5 N menunjukkan aktiviti semua enzim yang diuji dan pergerakan gigi kanin adalah tidak signifikan (p>0.05). Ujian korelasi Pearson's mendapati tiada korelasi di antara aktiviti spesifik enzim LDH, AST, TRAP dan ALP dengan jumlah enzim dalam GCF, mengindikasikan tidak semua enzim yang terhasil semasa pergerakan gigi kanin adalah enzim yang aktif. Tiada perbezaan aktiviti spesifik enzim dan jarak pergerakan gigi yang signifikan antara daya 1.0 N dengan 1.5 N yang diberikan. Profil aktiviti spesifik enzim dengan daya 1.5 N menunjukkan inflamasi dan kematian sel berlaku lebih awal berbanding daya 1.0 N. Di samping itu, daya 1.5 N juga didapati melambatkan proses penyerapan dan pembentukan tulang. Oleh itu, dicadangkan daya 1.0 N memadai untuk menggerakkan gigi kanin dalam rawatan ortodontik menggunakan SLB. Enzim LDH, AST dan TRAP daripada GCF didapati boleh digunakan sebagai penanda biologi untuk mengawasi perkembangan selular semasa pergerakan gigi kanin dalam rawatan ortodontik menggunakan SLB.,Orthodontic treatment caused changes in the activity of biological markers that involved processes such as inflammation (lactate dehydrogenase; LDH), necrosis (aspartate aminotransferase; AST), bone resorption (tartrate resistant acid phosphatase; TRAP) and bone formation (alkaline phosphatase; ALP). Objectives of this study were to determine the profile of biomarkers' activities and the correlation with total biomarkers presence. Gingival crevicular fluid (GCF) was collected from 19 orthodontic subjects before and during 5 weeks of orthodontic treatment. All subjects used self-ligating bracket (SLB) and orthodontic forces of 1.0 N or 1.5 N given either on the right or left side of maxillary arch randomly determined using toss of coin. Enzymes' specific activities and ELISA analysis for total enzyme determination were measured. Canine movement was measured using digital calliper. Statistical analysis was carried out using SPSS ver.20 software. Paired t-test analysis to compare enzyme activities between basal and during treatment showed significant increment (p<0.05) of LDH specific activity in the GCF at week 1, 2 and 3 (mesial; 1.0 N of force) and at week 1, 2, 3 and 4 (distal; 1.0 N of force) while from week 1 until 5 (mesial and distal; 1.5 N of force). Specific activity of AST showed significant increment (p<0.05) at week 4 and 5 (mesial and distal; 1.0 N of force) and week 3, 4 and 5 (mesial; 1.5 N of force) and at week 4 and 5 (distal; 1.5 N of force). TRAP specific activity was increased significantly (p<0.05) at week 5 (mesial; 1.0 N of force) and at week 3, 4 and 5 (distal; 1.0 N of force) whereas, there was no significant increment (p>0.05) for 1.5 N of force. Meanwhile, ALP specific activity did not showed any significant increment (p>0.05) at mesial and distal for both forces used (1.0 N and 1.5 N of force). In addition, when comparison made between 1.0 N and 1.5 N of force of specific activities (all enzymes measured) and canine movement, they showed no significant differences (p>0.05). Pearson's correlation test found no correlation between LDH, AST, TRAP and ALP enzymes' specific activities with total enzymes presence in the GCF, indicated that not all enzymes produced during tooth movement was an active enzyme. There were no significant differences in enzymes' specific activities and tooth movement when 1.0 N or 1.5 N of forces were applied. Profile of enzymes' specific activities with 1.5 N of force showed inflammation and cell death occurred earlier than 1.0 N of force. Furthermore, 1.5 N of force also delayed the bone resorption and formation processes. Therefore, 1.0 N of force is sufficient for canine retraction in orthodontic treatment using SLB. LDH, TRAP and AST enzymes from the GCF can be used as biomarkers for monitoring cellular development during orthodontic tooth movement by SLB.,Master | |
| dc.language.iso | may | |
| dc.publisher | UKM, Bangi | |
| dc.relation | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
| dc.rights | UKM | |
| dc.subject | Profil penanda biologi | |
| dc.subject | Rawatan ortodontik | |
| dc.subject | Braket swa-peligaturan | |
| dc.subject | Cecair krevis gingiva | |
| dc.subject | Gingival fluid. | |
| dc.title | Implikasi terhadap profil penanda biologi dalam cecair krevis gingiva manusia hasil rawatan ortodontik menggunakan braket SWA-Peligaturan | |
| dc.type | theses | |
| dc.format.pages | 132 | |
| dc.identifier.callno | RK410 .N846 2014 | |
| dc.identifier.barcode | 001674 | |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_81486+SOURCE1+SOURCE1.0.PDF Restricted Access | 2.51 MB | Adobe PDF |  View/Open |
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