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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Dr. Mohd Firdaus Raih | |
| dc.contributor.author | Sri Devan ( P53392) | |
| dc.date.accessioned | 2023-09-25T09:24:07Z | - |
| dc.date.available | 2023-09-25T09:24:07Z | - |
| dc.date.issued | 2013-10-01 | |
| dc.identifier.other | ukmvital:81280 | |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/463394 | - |
| dc.description | Interaksi RNA nukleotida memainkan peranan penting samada dalam menstabilkan struktur tiga-dimensi RNA atau fungsi lain yang khusus. Dalam kajian ini, interaksi RNA nukleotida telah dikenalpasti dan dianalisa secara in siliko. Tumpuan diberikan kepada struktur ribosuis bagi memahami fungsi dan tahap keterpeliharaan interaksi RNA nukleotida. Ribosuis merupakan unsur pengawalaturan RNA yang membentuk struktur 3D yang kompleks dalam menjalankan fungsi biologinya. Tujuh jenis interaksi RNA nukleotida (interaksi RNA 3 bes, A-minor, zip ribosa, interaksi reseptor-gegelung, interaksi gegelung-gegelung, pseudoknot dan kink-turn) telah dikenalpasti dalam sepuluh kelas ribosuis yang berlainan. Sebanyak 61 interaksi RNA nukleotida unik telah ditemui dalam struktur-struktur ribosuis rujukan yang mewakili sepuluh kelas ribosuis. Interaksi A-minor dan interaksi RNA 3 bes kerap ditemui di dalam struktur ribosuis; dan didapapti terlibat di dalam interaksi bes jarak-jauh yang membantu menghubungkan kawasan yang berjauhan di dalam struktur ribosuis serta membantu proses pelipatan struktur ribosuis. Tiga variasi interaksi A-minor yang dikenali sebagai interaksi G-minor telah ditemui dalam ribosuis S-adenosilmetionina- 1 (SAM-I) dan flavin mononukleotida (FMN). Ketiga-tiga interaksi ini terlibat dalam mengenalpasti kumpulan berfungsi ligan. Hasil penjajaran jujukan yang telah dilakukan ke atas ketiga-tiga interaksi ini menunjukkan bahawa ianya terpelihara secara sepenuhnya. Beberapa contoh variasi interaksi RNA nukleotida yang lain turut ditemui di dalam ribosuis seperti interaksi reseptor-gegelung-penta di dalam ribosuis lisina dan interaksi gegelung-gegelung yang diperantarakan oleh gegelung-T di dalam ribosuis FMN. Kebanyakan interaksi RNA nukleotida didapati mempunyai tahap keterpeliharaan yang tinggi dalam penjajaran jujukan-struktur ribosuis. Ini mencadangkan interaksi-interaksi yang mungkin penting bagi menstabilkan struktur 3D ribosuis atau penting bagi fungsi biologi ribosuis seperti pengawalaturan pengeskpresan gen. Hasil kajian ini boleh digunakan untuk lebih memahami fungsi dan tahap keterpeliharaan motif struktur tertier RNA dan seterusnya menyumbang kepada bidang lain yang berkaitan seperti peramalan struktur RNA dan kejuruteraan RNA buatan.,RNA nucleotide interactions play a vital role to either stabilize the formation of RNA 3-dimensional structures or to provide important biological function to RNA molecule. In order to better understand their roles and degree of sequence conservation of RNA nucleotide interactions, an in silico approach was used to search and analyze these interactions in riboswitch structures. Riboswitches are RNA regulatory elements that fold in complex 3D structures to perform their biological function. Seven different RNA nucleotide interactions (base-triple, A-minor, ribose zipper, loop-receptor interaction, loop-loop interaction, pseudoknot and kink-turn) were searched in ten distinct classes of riboswitches. A total of 61 unique RNA nucleotide interactions were found in the reference structures representing 10 distinct classes of riboswitches. Both base-triples and A-minor interactions were commonly found in riboswitches. These interactions are involved in long-distance base interactions linking distant regions in the riboswitch structures as well as assisting RNA folding. Three variants of A-minor interactions known as G-minors were found in S-adenosylmethionine-I (SAM-I) and flavin mononucleotide (FMN) riboswitch where they appear to be involved in the recognition of the respective ligand's functional groups. All G-minors were fully conserved in the alignments. In addition, other variants of known RNA nucleotide interactions were found in lysine riboswitch (pentaloop-receptor interaction) and in FMN riboswitch (loop-loop interaction mediated by a T-loop). Many of these interactions were highly conserved in the sequence-structure alignments, suggesting that these interactions might be crucial for the structural stability or biological function of riboswitches such as regulation of gene expression. The knowledge obtained from this study can be used to better understand their functions and sequence conservation/diversity of RNA tertiary motifs. The knowledge could be applied in other related fields such as RNA structure prediction and RNA synthetic biology.,Master | |
| dc.language.iso | may | |
| dc.publisher | UKM, Bangi | |
| dc.relation | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
| dc.rights | UKM | |
| dc.subject | Interaksi RNA nukleotida | |
| dc.subject | Struktur ribosuis | |
| dc.subject | Tahap keterpeliharaan | |
| dc.subject | RNA-protein interactions. | |
| dc.title | Analisis in siliko interaksi nukleotida berikat hidrogen dalam struktur tiga dimensi (3D) RNA | |
| dc.type | theses | |
| dc.format.pages | 118 | |
| dc.identifier.callno | QP623.7 .S696 2013 | |
| dc.identifier.barcode | 001668 | |
| Appears in Collections: | Faculty of Science and Technology / Fakulti Sains dan Teknologi | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_81280+SOURCE1+SOURCE1.0.PDF Restricted Access | 37.55 MB | Adobe PDF |  View/Open |
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