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Title:	Physiochemical properties of Saudi extra virgin olive oil and black cumin seed oil and effect of their mixture on eczema induced mice
Authors:	Nesrain Abduljaleel Saeed Qasim Farhan (P54735 )
Supervisor:	Jumat Salimon, Professor Dr.
Keywords:	Olive oil Seed oil
Issue Date:	Mar-2014
Description:	xtra virgin olive oil and black cumin seed oil (Habbatus sauda) are valueable essential oils. This work reports the study of physiochemical properties of Saudi extra virgin olive oil (EVOO), n-hexane extracted Saudi black cumin seed oil (BCSO) and mixture of EVOO with BCSO (3:1 v/v ratio). The result showed significant level of extracted black cumin seed oil - about 43.7%. All the oil samples show high saponification values and low unsaponification values. The investigation of iodine values show higher degree of unsaturation for BCSO compare to EVOO and EVOO-BCSO. The squalene of EVOO and volatile compounds of BCSO have been extracted using steam distillation method and analyzed using gas chromatography mass spectrometer (GC/MS). The results show that squalene content of EVOO is higher compared to that of EVOO-BCSO.The volatile compounds such as p-cymene and thymoquinone are high in BCSO and EVOO-BCSO. Other compounds such as 3,5-dimethyl cyclohexanol, α-thujene, carvacrol, peonol and longifolene are also present. Vitamin E is analyzed using High performance liquid chromatography (HPLC)-fluorescence method. The concentrations of vitamin E are 242, 451 and 400 ppm for EVOO, BCSO and EVOO-BCSO respectively. Gas Chromatography showed that EVOO has high content of oleic acid (72.2%), while BCSO has high content of linoleic acid (59.8%). Consequently, the mixture of both oils show significant level of both oleic (56%) and linoleic acids (27.9%). HPLC results show that major triacylglycerols (TAGs) of EVOO are 1,2,3-trioleylglycerol (OOO)35.2±0.3%, 1,2 -dioleoyl- 3- linoleoylglycerol (OOL) 29.03±0.2%, 1- palmitoyl -2,3 - dilinoleoylglycerol (PLL) 14.9±0.7% and 1,3-dipalmitoyl-2-oleoylglycerol (POP) 14.3±0.4% whereas TAGs of BCSO are 1-oleoyl -2,3-dilinoleoylglycerol (OLL) 37.7±0.4%, 1,2,3-trilinoleylglycerol (LLL) 35.9±0.3%, PLL 6.7±0.9%, OOO 6.4±0.5% and OOL 5.7±1.1%. TAGs of EVOO-BCSO mixture is similar to their original oils. The Fourier Transform Infrared Spectroscopy (FTIR) spectra show no significant differences among studied oils. Due to the predominant composition of EVOO-BCSO mixture, its effect on experimentally induced eczema on mice skin has been tested. The biological test showed that the skin condition of mice with eczema is improved significantly from the first week of treatment. Consequently, the EVOO-BCSO that contains a mixture of compounds from different oils appears to be useful in the treatment of eczema. Hereby, it is suitable to show that the results of this study can be helpful in the design of effective natural methods of therapy for eczema on mice skin with low cost, short time and without side effects.,Master/Sarjana
Pages:	124
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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