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Title:	Population of soil fungi during field biodegradation of shreddedd oil palm trunk using fungal consortia in an oil palm plantation
Authors:	Norsam Tasli Mohd Razali (P34961)
Supervisor:	Siti Ramlah Ahmad Ali, Dr.
Keywords:	Biodegradation Oil Palm Soil microbiology
Issue Date:	8-Mar-2012
Description:	The study was done to observe the changes in the number of soil fungi colonies during field biodegradation of shredded oil palm trunk which was inoculated with fungi consortia and to identify the fungi degrading-cellulose isolated from the soil. Analysis of soil fungi population was done using molecular approach and culture plate method. All soil samples were collected at Malaysian Palm Oil Board (MPOB) Keratong, Pahang. Soil samples before fungal treatment were collected from April 2006 to June 2006 while; samples after treatment were collected from July 2006 to June 2007. Each of the plot was treated with different treatments, Treatment 1 (T1) consists of Trichoderma harzianum and brown-rot (SK8/11), Treatment 2 (T2); Trichoderma harzianum and SK7/5, Treatment 3 (T3); Trichoderma reseei and SK8/11, Treatment 4 (T4); Trichoderma reseei and SK7/5 and control plot (T5), without addition any degrading fungi. Culture plate method had indicated that number of soil fungi was high at first month after degradation (MAD), the number of soil fungi were increased to tenfold for all plots with the average of 7.25 x104, 6.5x104, 6.5 x104, 6.7 x104 and 7.25 x104 CFUg-1soil for plots T1, T2, T3, T4 and T5, respectively. DGGE results showed the average number of dominant bands was highest at 1 MAD at 11, 10, 10, 9 and 19 bands for plots T1, T2, T3, T4 and T5, respectively. Dendogram showed the similarity percentage had increased to 51% for 1 month sample and start to decrease at 49% during 2 MAD. DGGE indicates that the number of dominant bands was highest at 1 MAD to 6 MAD but the number of fungal species was lowest or less diversified. On the contrary from 9 MAD to 12 MAD the fungal count was lowest but population of fungi was more diversified. Isolation and identification of soil fungi showed 25 isolates of common soil fungi that belong to seven different genus, which were Aspergillus sp., Penicillium sp., Peacilomyces sp., Cunninghamella sp., Trichoderma sp., Mucor sp. and Fusarium sp.. Screening of cellulolytic fungi was done by culturing the 25 isolates of fungi in Mandel's agar. The result showed only 4 isolates were able to grow in Mandel's agar after 4 days incubation at 37oC. These fungi were identified as Trichoderma reesei, Trichoderma harzianum, Aspergillus niger and Aspergilus flavus and were selected for cellulase assay using carboxymethylcellulose (CMC) and p-nitrophenyl-β-d-glucoside (pNPG) as substrate. Enzyme assay showed Trichoderma sp. produced endo-ß-glucanase and exo-ß-glucanase which can be measured by CMCase activities. CMCase activities were maximum in day 3, which was at 0.35 IU/mL, 0.35 IU/mL, 0.13 IU/mL and 0.12 IU/mL for Trichoderma reesei, Trichoderma harzianum, Aspergillus niger and Aspergillus flavus, respectively. ß-glucosidase activity after 5 days incubation showed a value of 0.62 IU/mL, 0.42 IU/mL, 0.94 IU/mL and 0.84 IU/mL for Trichoderma reesei, Trichoderma harzianum, Aspergillus niger and Aspergillus flavus, respectively.,Certification of Master's/Doctoral Thesis" is not available
Pages:	121
Call Number:	QR111.N677 2012 tesis
Publisher:	UKM, Bangi
Appears in Collections:	Faculty of Science and Technology / Fakulti Sains dan Teknologi

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