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https://ptsldigital.ukm.my/jspui/handle/123456789/457977Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Chua Kien Hui, Dr. | - |
| dc.contributor.author | Wan Safwani Wan Kamarul Zaman (P42716) | - |
| dc.date.accessioned | 2023-09-13T01:48:57Z | - |
| dc.date.available | 2023-09-13T01:48:57Z | - |
| dc.date.issued | 2012-02-10 | - |
| dc.identifier.other | ukmvital:85039 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/457977 | - |
| dc.description | Adipose tissue is a source of multipotent adult stem cells. They are easily harvested and can be found in abundance. However, the safety and efficacy issues concerning the use of adult stem cells after long-term or extensive in vitro manipulation have not been well established yet. It is important to address these issues to ensure the safety of stem cell based therapy. The aim of this study was to investigate the multipotentiality of human adipose-derived stem cells (hADSCs) in long-term culture. The hADSCs were harvested, isolated and culture-expanded from lipoaspirate sample. Morphological, growth kinetics, surface marker properties, stemness gene expression profile, differentiation ability, senescence profile and tumorigenic potential evaluation were performed on long-term cultured hADSCs at P5, P10, P15 and P20. The growth kinetics of hADSCs in long-term culture declined significantly at later passage (P15 and P20). Data from quantitative RT-PCR showed that their stemness gene expressions decreased after long-term culture. While data on surface marker expression showed that hADSCs in long-term culture may have differentiated into more specific cells. These changes were observed in hADSC after P10. The hADSCs were able to differentiate into adipogenic, osteogenic, neurogenic but not cardiogenic cells, while the study on the senescence profile and tumorigenic potential of hADSCs revealed that hADSCs in long-term culture may favour senescence. The cell morphology exhibited the characteristics of senescence and DNA damage was more apparent at P15 and P20. Furthermore, data from the cell cycle analysis showed most of hADSCs were in the G0/G1 phase at all passages. There were no significant changes in the mean TRF length and relative telomerase activity (RTA) of long-term culture hADSCs at all passages. The study performed on nude mice revealed that there was no growth of lump even after 4 months of observation when hADSCs were injected subcutaneously. In conclusion, hADSCs can be used up to P20 without any significant changes in their genomics. However, due to the significant changes in their stemness properties and differentiation ability after P10, we suggest that P10 be the 'cut-off' point for cell expansion in the clinical settings. This knowledge may be incorporated as part of the Good Manufacturing Practice (GMP) guidelines for stem cell based therapy.,Master / Sarjana | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Kuala Lumpur | - |
| dc.relation | Faculty of Medicine / Fakulti Perubatan | - |
| dc.rights | UKM | - |
| dc.subject | Biochemistry | - |
| dc.title | Long-term multipotential of stem cells isolated from human adipose tissue | - |
| dc.type | theses | - |
| dc.format.pages | 299 | - |
| dc.identifier.callno | QU20.W244L 2012 9 HUKM | - |
| dc.identifier.barcode | 002106 | - |
| Appears in Collections: | Faculty of Medicine / Fakulti Perubatan | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_85039+SOURCE1+SOURCE1.0.PDF Restricted Access | 6.83 MB | Adobe PDF |  View/Open |
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