Please use this identifier to cite or link to this item:
https://ptsldigital.ukm.my/jspui/handle/123456789/457942| Title: | Germ tube positive yeasts in vaginal samples : issues in identification and determination of antifungal susceptibility profiles among phenotypically unusual isolates |
| Authors: | Atta Yazdanpanah (P 56937) |
| Supervisor: | Tzar Mohd Nizam bin Khaithir, Dr. |
| Keywords: | Germ Yeasts Vaginal Antifungal Phenotypically Microbiology |
| Issue Date: | 18-Sep-2013 |
| Description: | Candida albicans is the most frequently identified species of yeast isolated from vaginal samples. However, over the past few years non-albicans pathogens such as C. dubliniensis and the newly identified C. africana have been associated with vaginal infections in humans. The present study was conducted to identify these closely related germ tube positive yeasts from patients with positive high vaginal swab samples. A total of 98 germ tube positive yeast samples isolated from high vaginal swabs were studied over a 12 month period. Isolates were examined with different phenotypic methods including ability to grow at 42°C and 45°C on Sabouraud dextrose agar (SDA), color development on CHROMagar Candida medium, chlamydospore production on corn meal agar at 25°C and API ID 32C system. All the samples were molecularly confirmed by using a single pair of primers targeting the hyphal wall protein 1 (HWP1) gene. Of all the isolates studied, 97 were molecularly confirmed as C. albicans and one isolate was identified as C. dubliniensis. The molecular method used in this study was an accurate and useful tool for discriminating C. albicans, C. dubliniensis and C. africana. The C. dubliniensis isolate showed no resistance to antifungal agents when tested with the Sensititre® YeastOne® in vitro diagnostic test. Based on the latest documentations to date; this study is the first well documented report of C. dubliniensis identified with molecular methods in Malaysia from high vaginal swab samples, and the first epidemiological study to deal with C. africana in vaginal samples in Southeast Asia and especially Malaysia.,Master/Sarjana |
| Pages: | 104 |
| Call Number: | QW20.5.Y11g 2013 9HUKM |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Medicine / Fakulti Perubatan |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_74654+Source01+Source010.PDF Restricted Access | 1.66 MB | Adobe PDF |  View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.