Please use this identifier to cite or link to this item: https://ptsldigital.ukm.my/jspui/handle/123456789/457697
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dc.contributor.advisorFatihah Suja, Dr.-
dc.contributor.authorFazli Rahim (P42467)-
dc.date.accessioned2023-09-12T09:12:16Z-
dc.date.available2023-09-12T09:12:16Z-
dc.date.issued2012-09-05-
dc.identifier.otherukmvital:115183-
dc.identifier.urihttps://ptsldigital.ukm.my/jspui/handle/123456789/457697-
dc.descriptionA feasibility treatment study was conducted on Tapis light crude oil-contaminated soil in order to assess potential use of bioremediation technology. The study objective was to evaluate and compare the optimum bioremediation treatment conditions using nutrient biostimulation and/or microbial bioaugmentation using researched indigenous microbial consortium MC3 (Acinetobacter sp strain 12T and Pseudomonas sp strain 14T). Field assessment data for physical, chemical, biological and geological conditions were obtained from previous site assessments. The Site Specific Target Limit (SSTL) for total petroleum hydrocarbon was obtained from Risk-Based Corrective Action (RBCA) software. The bioremediation study was continued in laboratory studies (microcosm and soil column setup) and field demonstration study. In laboratory study, treatments studied included biostimulation with nutrients using either mineral salt media (MSM) or combination of NH4NO3, Na2HPO4 and KH2PO4 as NPK sources, aeration using either Oxygen Releasing Compound (ORC™) or compressed air at 0.5 to 2.5 L/hr. Microcosm test was conducted inside microcosm glass tanks sized 20 cm × 20 cm × 10 cm whilst the soil column was fabricated from 70% clear PVC and sized with 15 cm ID and height of 80 cm. Eight soil sampling ports and three stainless steel air distributor ring were installed at different depth. In the field test, microbial consortium MC3 was tested at 1m × 1m test plots at actual Tapis crude oil-contaminated soil site. Petroleum hydrocarbons (TPH) and total heterotrophic counts were measured as bioremediation indicator. In microcosm experiment, combination of bioaugmentation (MC3 of 10% inoculum size) and biostimulation (MSM addition) yielded the highest TPH degradation of 79%, followed by biostimulation only (67%) and bioaugmentation only (41%). Another similar experiment using NPK nutrients was conducted and TPH degradation of 82% was achieved for the same combination as compared to 63% TPH degradation in Control. In soil column experiment, TPH degradation performance in top soil (0 - 40cm) was as follows: Column A (Bioaugmentation + Nutrient) > Column B (Bioaugmentation + Nutrient + ORC) > Column C (Bioaugmentation + Nutrient + Low Air Flow) > Control (+) > Control (-) > Column D (Bioaugmentation + Nutrient + High Air Flow). For bottom soil (40-80 cm), the TPH degradations were as follows: Column B (Bioaugmentation + Nutrient + ORC) > Column C (Bioaugmentation + Nutrient + Low Air Flow) > Column D (Bioaugmentation + Nutrient + High Air Flow) > Column A (Bioaugmentation + Nutrient) > Control (+) > Control (-). Field demonstration study revealed TPH degradation was recorded at 91% in top soil as compared to 62% in bottom soil. TPH biodegradation kinetic rates were 0.021 day-1 (microcosm), 0.023 day-1 (soil column) and 0.012 to 0.034 day-1 (field). Bioaugmentation with MC3 consortium had increased TPH biodegradation when combined with nutrient and oxygen stimulations. The use of ORC™ in soil column experiments had further enhanced TPH biodegradation process for top and bottom soil. Diesel range (C13-C28) fractions were more biodegraded as compared to residual range (C29-C36) in all experiments. TPH biodegradation process was related to high total heterotrophic count (THC) of more than 106 CFU/g of indigenous and inoculated microbes.,“Certification of Master’s/Doctoral Thesis” is not available,Master Science-
dc.language.isoeng-
dc.publisherUKM, Bangi-
dc.relationFaculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina-
dc.rightsUKM-
dc.subjectSoil remediation-
dc.subjectBioremediation-
dc.subjectUniversiti Kebangsaan Malaysia -- Dissertations-
dc.subjectDissertations, Academic -- Malaysia-
dc.titleTreatment of crude oil contaminated soil using bioremediation processes in laboratory and field tests-
dc.typetheses-
dc.format.pages154-
dc.identifier.callnoTD192.5.F349 2012 3 tesis-
dc.identifier.barcode002528(2012)-
Appears in Collections:Faculty of Engineering and Built Environment / Fakulti Kejuruteraan dan Alam Bina

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