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https://ptsldigital.ukm.my/jspui/handle/123456789/457434| Title: | An in-vitro study of anti-streptococcus mutans effects of virgin coconut and olive oils |
| Authors: | Halimah binti Mohamed Noor (P72956) |
| Supervisor: | Alida Mahyuddin, Dr. |
| Keywords: | Mouthwash Dental caries Virgin coconut Olive oils Dissertations, Academic -- Malaysia |
| Issue Date: | 21-Feb-2017 |
| Description: | The natural products are sought to be an alternative mouthwash in controlling the dental caries especially in children. The objectives of this study were to determine the minimum inhibitory concentration (MIC) of the virgin coconut and virgin olive oils in inhibiting Streptococcus mutans in a various salivary pH, to predict the effect of oil concentration and salivary pH on bacterial inhibition activity by the oil and to compare anti-Streptococcus mutans effect between coconut oil, olive oil and chlorhexidine in various salivary pH. Methodologically, the Streptococcus mutans strain was cultivated in brain heart infusion agar. The inhibition of Streptococcus mutans was tested on 96- well microtiter plate. The growth of bacteria was enhanced with 1% glucose, and artificial saliva. The artificial saliva was prepared in six different pH i.e.; pH 6.0, 5.7, 5.4, 5.1, 4.8, and 4.5. The bacterial inoculum were incubated at 370C for 48 hours to allow the formation of a biofilm. The bacterial biofilm were then exposed to VCO and VOO in serially diluted concentration (100%, 50%, 25%, 12.5%, 6.25%, 3.13%, 1.57% and 0.79%). The inhibition was measured under a microplate reader. As the result, the MIC value for both oil were read according to each salivary pH. The oil concentration which caused bacterial inhibition >80% was the MIC. The bacterial inhibition by the oils can be predicted by the oil concentration and salivary pH (p < 0. 01). Then, factorial between group analysis of variance (ANOVA) was carried out to compare the anti- Streptococcus mutans among the VCO, VOO and chlorhexidine. VCO at concentration of >25% showed significantly more superior antibacterial activity against Streptococcus mutans than VOO (MIC 100%) and chlorhexidine (MIC 0.12%), even in acidic environment (pH 4.5) (p < 0.05). VCO can be an alternative mouthwash to chlorhexidine especially in children because it is non-toxic, easily obtainable, and edible.,Doktor Pergigian Klinikal |
| Pages: | 98 |
| Call Number: | WU20.5.H173i 2017 9 |
| Publisher: | UKM, Kuala Lumpur |
| Appears in Collections: | Faculty of Dentistry / Fakulti Pergigian |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_99810+Source01+Source010.PDF Restricted Access | 1.65 MB | Adobe PDF |  View/Open |
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