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https://ptsldigital.ukm.my/jspui/handle/123456789/457427Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Ahmad Tarmidi Sailan, Dr. | - |
| dc.contributor.author | Nyi Mas Siti Purwaningsih binti Md Hasan Basari (P74238) | - |
| dc.date.accessioned | 2023-09-12T04:06:45Z | - |
| dc.date.available | 2023-09-12T04:06:45Z | - |
| dc.date.issued | 2017 | - |
| dc.identifier.other | ukmvital:98532 | - |
| dc.identifier.uri | https://ptsldigital.ukm.my/jspui/handle/123456789/457427 | - |
| dc.description | Ninety-five percent of oral cancer are classified as oral squamous cell carcinoma (OSCC). The p16 and p53 are protein in human cell cycle regulation that can play a role as a tumour suppressor gene. Human papillomavirus was known as one the aetiology factor that can cause OSCC. The purpose of the research is to detect p16, p53 and HPV expressions in potentially malignant disorders (OPMD) and OSCC using immunohistochemistry (IHC). A total of 118 formalin-fixed paraffin embedded tissue were selected for test group, OSCC cases (n=46), OPMD cases (n=32), oral epithelial hyperplastic (OEH) (n=31) and control group normal oral mucosa (NOM) (n=9). The cases were retrieved from the archives of the Oral Medicine Oral Pathology Laboratory (OMOP), Faculty of Dentistry, UKM and Stomatology Unit, Institute Medical Research (IMR), Kuala Lumpur and Tuanku Mizan Military Hospital, Kuala Lumpur. The IHC staining was manually performed using HPV (1:50), p16 (1:1000) and p53 Ready to use (RTU) antibodies following manufacturer’s instruction and assessed semi-quantitatively (positivity and staining intensity) between test and control groups. Positive and negative controls were used to validate the IHC run. All data were then analysed using SPSS version 23.0 and p<0.05 were considered significant. The p16 were found positive in OSCC (97.83%), OPMD (84.38%), OEH (83.87%) and only 12.50% in control group (NOM). There is significantly higher p16 positivity in OSCC compared to normal mucosa (p<0.001). There are high statistically significant differences in p16 expression between NOM and OPMD (p<0.001), as well as NOM and OEH (p<0.001). Similarly, there are significant differences in p53 expression between NOM and OSCC (p<0.01) and also between OPMD and OSCC (p<0.05). In the present study, the p16 and the p53 positive expression were increased following the degree of malignancy. The finding suggests that p16 and p53 can be used as a potential marker for oral malignancy detection. HPV found to be one of important aetiological factor in oral cancer in Malaysia.,Ijazah Sarjana Sains Pergigian | - |
| dc.language.iso | eng | - |
| dc.publisher | UKM, Kuala Lumpur | - |
| dc.relation | Faculty of Dentistry / Fakulti Pergigian | - |
| dc.rights | UKM | - |
| dc.subject | Oral squamous cell carcinoma | - |
| dc.subject | Oral cancer | - |
| dc.subject | Dissertations, Academic -- Malaysia | - |
| dc.title | Detection of human papillomavirus, P16 and P53 in oral potentially malignant disorders and oral squamous cell carcinoma | - |
| dc.type | theses | - |
| dc.format.pages | 99 | - |
| dc.identifier.callno | QW165.5.P2 N994d 2017 9 | - |
| Appears in Collections: | Faculty of Dentistry / Fakulti Pergigian | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ukmvital_98532+Source01+Source010.PDF Restricted Access | 248.97 kB | Adobe PDF |  View/Open |
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