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Title:	A cytotoxicity study of newly developed beta titanium alloys on human gingival fibrolasts
Authors:	Nor Haliza Mat Baharin (P57826)
Supervisor:	Masfueh Razali, Dr.
Keywords:	Aloi titanium Sitotoksik Gingivitis Dentistry Dissertations, Academic -- Malaysia Universiti Kebangsaan Malaysia -- Dissertations
Issue Date:	6-Mar-2014
Description:	Three types of new β-titanium alloys has been developed by Faculty of Engineering and Built Environment UKM for dental use and the biomaterials need to be tested for cytotoxicity prior to clinical study. Therefore, the aim of this study is to investigate the effect of Ti-10Mo-10Cr, Ti-10Cr and Ti-10Mo alloys on human gingival fibroblasts. Each type of alloy was immersed in growth medium for 1, 14 and 21 days. The medium was analysed by ICP-MS and also used as treatment medium to test the cell viability. The fibroblasts were also exposed directly to all β-titanium alloys for 1, 3 and 7 days. The cell viability was assessed with MTT by calculating the absorbance. The cell viability and the concentrations of element release were also compared. Six metal elements were detected with ICP-MS, with molybdenum showing an unconstant release from Ti-10Mo-10Cr. Upon indirect exposure of the biomaterials to the fibroblasts, there was no significant difference in cell viability for all type of titanium alloys. With direct exposure to the alloys, Ti-10Mo and Ti-10Cr showed reduced cell viability after 7 days of direct contact. Comparing all the cell viability after direct exposure to β-titanium alloys, significant differences were obtained on duration of exposure (p=0.02). In conclusion, the newly developed β-titanium alloys are non-cytotoxic to human gingival fibroblasts. Some growth inhibitory effect was seen in fibroblasts with direct contact to the alloys but it did not reach toxicity level.,Tiga jenis aloi titanium β baru telah dihasilkan oleh Fakulti Kejuruteraan dan Alam Bina, UKM untuk kegunaan pergigian. Biobahan tersebut perlu menjalani ujian sitotoksik sebelum ujian klinikal. Oleh itu, kajian ini dilakukan bertujuan untuk mengkaji kesan aloi Ti-10Mo-10Cr, Ti-10Cr dan Ti-10Mo terhadap sel fibroblas. Media pertumbuhan yang telah direndam setiap jenis aloi di dalamnya digunakan sebagai media rawatan untuk mengukur tahap viabiliti sel dan sebahagiannya digunakan untuk mengenalpasti kehadiran elemen yang larut-lesap melalui kajian ICP-MS. Fibroblas juga telah dibiarkan membiak secara terus dengan aloi-aloi kajian selama 1, 3 dan 7 hari. Kadar viabiliti sel diukur dengan mengira kadar penyerapan assay MTT. Viabiliti sel dan kepekatan elemen yg didapati dari rendaman aloi juga dibandingkan. Enam elemen metal telah dikesan melalui analisis ICP-MS dan didapati molibdenum mempunyai kepekatan yang tinggi dari hasil rendaman aloi Ti-10Mo-10Cr. Tiada aloi didapati menunjukkan kesan toksik dengan ketara apabila perbandingan viabiliti sel yang didedahkan secara tidak langsung terhadap biomaterial tersebut dibuat. Dengan pendedahan aloi secara terus terhadap fibroblas, Ti-10Mo dan Ti-10Cr menunjukkan penurunan viabiliti sel selepas 7 hari. Perbandingan viabiliti sel selepas terdedah kepada aloi Ti-10Mo dan Ti-10Cr menunjukkan perbezaan statistik yang ketara terhadap tempoh pendedahan (p=0.02). Kesimpulannya, kesemua aloi β-titanium yg dikaji adalah tidak toksik terhadap sel fibroblas. Kesan perencatan pertumbuhan dapat dilihat dengan sel yang didedahkan secara terus dengan aloi namun ia tidak mencapai tahap yang boleh dikategorikan sebagai toksik.,Doctor in Clinical Dentistry (Periodontology),Three types of new β-titanium alloys has been developed by Faculty of Engineering and Built Environment UKM for dental use and the biomaterials need to be tested for cytotoxicity prior to clinical study. Therefore, the aim of this study is to investigate the effect of Ti-10Mo-10Cr, Ti-10Cr and Ti-10Mo alloys on human gingival fibroblasts. Each type of alloy was immersed in growth medium for 1, 14 and 21 days. The medium was analysed by ICP-MS and also used as treatment medium to test the cell viability. The fibroblasts were also exposed directly to all β-titanium alloys for 1, 3 and 7 days. The cell viability was assessed with MTT by calculating the absorbance. The cell viability and the concentrations of element release were also compared. Six metal elements were detected with ICP-MS, with molybdenum showing an unconstant release from Ti-10Mo-10Cr. Upon indirect exposure of the biomaterials to the fibroblasts, there was no significant difference in cell viability for all type of titanium alloys. With direct exposure to the alloys, Ti-10Mo and Ti-10Cr showed reduced cell viability after 7 days of direct contact. Comparing all the cell viability after direct exposure to β-titanium alloys, significant differences were obtained on duration of exposure (p=0.02). In conclusion, the newly developed β-titanium alloys are non-cytotoxic to human gingival fibroblasts. Some growth inhibitory effect was seen in fibroblasts with direct contact to the alloys but it did not reach toxicity level
Pages:	65
Call Number:	WU240.N8253c 2014 9 tesis
Publisher:	UKM, Kuala Lumpur
Appears in Collections:	Faculty of Dentistry / Fakulti Pergigian

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