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Title:	Development of palm glyceryl monocaprylate nanoemulsion with antibacterial properties for wound healing application
Authors:	Chua Siaw Kim (P89753)
Supervisor:	Ng Shiow Fern, Assoc. Prof. Dr.
Keywords:	Anti-Bacterial Agents Wounds Universiti Kebangsaan Malaysia -- Dissertations Dissertations, Academic -- Malaysia
Issue Date:	30-Mar-2022
Description:	Palm glyceryl monocaprylate (GMCY), a medium chain monoglycerides derived from palm kernel oil, has been reported to have antibacterial properties. However, there is a lack of study on the development of functional formulation using palm GMCY for wound application. In this study, the potential application GMCY as antibacterial nanoformulation for the treatment of infected wounds was explored. First, the antibacterial activity of GMCY was tested against three common wound bacteria, namely Escherichia coli, Staphylococcus aureus dan Pseudomonas aeruginosa using the broth dilution method. It was found that GMCY exhibited bactericidal activities against S. aureus and E. coli with the minimum bactericidal concentration of GMCY at 1% (w/w). Electron microscopy observation on the bacteria morphology demonstrated that increment in GMCY concentration caused more severe damaged to the cell membrane. Based on the results of nanoemulsion optimization study using response surface methodology (RSM), it was found that there was a direct correlation between GMCY concentration with the particle size and polydispersity index of the nanoemulsion. Through the RSM models developed, an optimized nanoemulsion containing 1% (w/w) GMCY (GMCY-NE) that has the characteristics of 120 nm in particle size, -16.67 mV in zeta potential value and 0.1 polydispersity index was obtained. The performance of GMCY-NE was evaluated through in vivo infected wound healing study using Sprague-Dawley rats. The healing rate was compared with nanoemulsion without GMCY (Blank-NE). Additionally, two nanoemulsions containing 0.05% (w/w) gentamicin sulfate, with (GMCY-GE-NE) and without (GENE) 1% (w/w) GMCY, were also prepared as comparison. The positive control of the study was a commercial cream containing 1% (w/w) gentamicin sulfate. Based on the results obtained, it was found that the wounds treated with GMCY-GE-NE and GMCY-NE healed fastest among all the treatment groups. Histological analysis of the recovered wounds also showed that GMCY-NE demonstrated better wound healing in terms of skin thickness where the recovered skin layers were found to be most similar to normal healthy skin. Formation of hair follicles was also more apparent in the GMCY-NE treated group. The findings of this study suggest that GMCY could be further explore for use in the treatment of infected wounds,Ijazah Sarjana Sains
Pages:	125
Publisher:	UKM, Kuala Lumpur
Appears in Collections:	Faculty of Pharmacy / Fakulti Farmasi

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