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Title:	Chemical constituents from Dillenia Serrata THUNB. (Dilleniaceae) and Syzygium polyanthum (WIGHT) WALP. (Myrtaceae) and their inhibitory effects on Prostaglandin E2 (PGE2) production and antioxidant activity
Authors:	Carla Wulandari Sabandar (P60220)
Supervisor:	Norizan Ahmat, Assoc. Prof. Dr.
Keywords:	Medicinal Plants Antioxidant activity Dilleniaceae Myrtaceae Dissertations, Academic -- Malaysia Universiti Kebangsaan Malaysia -- Dissertations
Issue Date:	17-Jun-2016
Description:	Dillenia serrata and Syzygium polyanthum are medicinal plants belonging to the family Dilleniaeae and Myrtaceace, respectively. Both plants are used traditionally to treat diseases involved in inflammation and oxidative stress. In this study, the MeOH extracts, fractions (petroleum ether, ethyl acetate, and methanol) and compounds from both plants were investigated on lipopolysaccharide (LPS)-induced prostaglandin E2 (PGE2) production in human whole blood by using a radioimmunoassay technique and antioxidant activity by using diphenyl-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. PGE2 is known as a mediator of inflammation. Screening of MeOH extracts of stem bark and root bark as well as fractions of the plants showed inhibitory effects on the production of PGE2 ranging from 15.92-73.86% at a concentration of 10 μg/mL. Four compounds isolated from D. serrata including betulinic acid (IC50 2.59 μM), 3-oxoolean-12-en-30-oic acid (1.54 μM), koetjapic acid (IC50 1.05 μM) and stigmasterol (IC50 1.25 μM) showed significant inhibition as compared with positive control, indomethacin (IC50 0.45 μM). Six compounds were isolated from the stem bark of S. polyanthum including stigmasterol, 3-hexyl-5-[methoxycarbonyl]benzoic acid, 3,3'-di-O-methylellagic acid, methyl gallate, daucosterol, and mixture of asiatic acid and arjunolic acid. Compound 3-hexyl-5-[methoxycarbonyl]benzoic acid was identified as a new compound from this plant. Out of these compounds, the mixture of asiatic and arjunolic acids was the most active inhibitor with IC50 of 0.052 μM, followed by daucosterol with IC50 of 0.078 μM, and they even more potent than positive control, indomethacin. In contrast, 3-hexyl-5-[methoxycarbonyl]benzoic acid and 3,3'-di-O-methylellagic acid showed no inhibition at all. Methyl gallate exhibited weak inhibitory effect of PGE2 production with IC50 of 33.62 μM. All active compounds showed dose dependent inhibitory effects on the PGE2 production. Two major compounds, betulinic acid and koetjapic acid were quantified in the crude extracts and fractions of D. serrata stem bark and root bark by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of betulinic acid (15.1%) and koetjapic acid (52.8%). The strong inhibition of the extracts and fractions on PGE2 production was suggested due to the presence of their major constituents, especially koetjapic and betulinic acids. The MeOH extracts, fractions, and compounds from both plants also exhibited DPPH radical scavenging activity and reductive action. Overall, the MeOH extracts and fractions of S. polyanthum plant parts showed promising DPPH radical scavenging activity, consistent with their reductive action, and this activity was strongly correlated with their phenolics content. Methyl gallate revealed as a potent antioxidant agent with IC50 value of 1.95 μM, comparable with ascorbic acid, trolox and gallic acid values. From this study, it is concluded that D. serrata and S. polyanthum are promising natural sources for anti-inflammatory drugs.,Master of Science
Pages:	209
Call Number:	QV20.5.C278c 2016 9 tesis
Publisher:	UKM, Kuala Lumpur
Appears in Collections:	Faculty of Pharmacy / Fakulti Farmasi

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