https://ptsldigital.ukm.my/jspui/handle/123456789/388901 2026-04-28T10:51:17Z https://ptsldigital.ukm.my/jspui/handle/123456789/783001 Title: Shelf life and safety evaluation of the dermal fibroblasts conditioned medium (DFCM) for future skin regeneration and rejuvenation Authors: Vinoothini A/P Karunnanithy (P115969) Abstract: Studies discovered that dermal fibroblasts conditioned medium (DFCM) as potential agent for skin regeneration and rejuvenation. However, the stability of these secreted proteins remains unknown. This study aimed to evaluate the stability of DFCM for skin regeneration and rejuvenation, focusing on storage conditions over time. Human dermal fibroblasts were cultured in serum-free fibroblast-specific medium (DFCM-FM) or keratinocyte- specific medium (DFCM-KM) for 72 hours, and the protein profile was analysed using liquid chromatography- mass spectrometry (LC-MS), Bicinchoninic Acid Protein Assay (BCA), and Enzyme-linked immunosorbent assay (ELISA). Then effects of DFCM on fibroblasts attachment, proliferation, migration, and cell characterisations were also assessed. DFCM-FM was formulated for skin care product and the physical and chemical stability evaluated. LC-MS identified 16 proteins in both DFCM, involved in skin remodelling and repair. Protein concentrations in DFCM-FM at 4 °C, -20 °C and -80 °C remained consistent up to 6 months. DFCM-KM stored at - 20 °C and -80 °C was stable for up to 6 and 12 months respectively. The collagen and fibronectin concentrations were more stable in DFCM-FM than in DFCM-KM, especially at lower storage temperatures. DFCM-FM enhanced fibroblasts attachment, proliferation, and migration over 6 months, while DFCM-KM was most effective at 1 month. Immunocytochemical staining revealed positive expression of collagen type I and Ki67 in both groups. Formulations of DFCM-FM (toner, serum, moisturizer, and mask) were stable under various temperature stress tests, maintaining appearance, pH, and viscosity. Microbiological and heavy metal contamination tests confirmed the formulations' safety. The study concludes that both DFCM exhibit promising storage stability under varying conditions, with DFCM-FM remaining stable for up to 6 months and DFCM-KM at -80 °C for up to 12 months. The formulations with DFCM-FM were confirmed to be safe and stable, highlighting their potential for clinical applications. 2026-04-02T00:00:00Z https://ptsldigital.ukm.my/jspui/handle/123456789/782859 Title: Kajian kepuasan pelanggan dan faktor-faktor yang mempengaruhinya di Jabatan Kecemasan & Trauma Hospital Sungai Buloh Authors: Muhammad Zulfadlil Azhim Ayub (P57576) Abstract: Pendahuluan: Satu kajian irisan lintang telah dijalankan untuk menentukan tahap kepuasan pelanggan di Jabatan Kecemasan & Trauma Hospital Sungai Buloh dari Februari 2012 hingga April 2012. Methodologi: seramai 288 responden telah diambil dengan menggunakan pensampelan mudah. Borang soal selidik berpandu telah digunakan untuk mengumpul data kajian. Data kajian dianalisis menggunakan SPSS versi 17.0. Keputusan: Majaroti daripada responden ialah wanita (54.5%) dan 79.2% daripada mereka telah berkahwin. Majaroti daripada responden daripada bangsa Melayu (56.3%), diikuti oleh bangsa Cina (14.2%), India (22.9%) dan lain-lain (6.6%). Lebih separuh (79.2%) daripada mereka berumur diantara 18 – 45 tahun dengan berpendidikan sekolah Menengah (63.9%) dan bekerja (78.5%). Secara keseluruhanya responden berpuashati dengan perkhidmatan kesihatan yang diberikan (92.4%). Kebanyakan responden berpuashati dengan aspek interpersonal (76.7%), kualiti teknikal (91.3%), asesibiliti (90.6%), efikasi (90.3%), persekitaran fizikal (97.2%), availabiliti (85.8%) dan caj perkhidmatan (88.5%). Manakala responden tidak berpuashati dengan penjagaan berterusan (94.1%) dan masa menunggu (76.7%). Hasil analisis bivariat menunjukkan hanya tiga faktor sahaja yang mempunyai perbezaan bererti (p<0.05) dengan kepuasan pelanggan, iaitu tahap jantina, bangsa dan pendidikan. Kesimpulan: Secara keseluruhanya responden berpuashati dengan perkhidmatan yang diberikan. Walaubagaimanapun dari segi masa menunggu dan penjagaan berterusan perlu diperbaiki. 2012-07-25T00:00:00Z https://ptsldigital.ukm.my/jspui/handle/123456789/782850 Title: Blood lead as a risk factor for myocardial infarction in Universiti Kebangsaan Malaysia Medical Center Authors: Rusna Meswari (P72412) Abstract: Lead is one of the pollutants that can cause adverse effects on human health. The exposure of lead has received much attention in some decade due to its non biodegradable properties. Blood lead measurement is the most convenient as well as the most feasible to indicate lead toxicity. The aim of this study was to assess the association between blood lead and myocardial infarction (MI). This is a hospital-based case-control study and was conducted at Universiti Kebangsaan Malaysia Medical Center. The case-control study enrolled 109 respondents; 71 cases and 38 non-cases of myocardial infarction. Research instruments include questionnaires on demographic factors (age, gender, and ethnicity), socioeconomic (education, household income, occupation), lifestyle (smoking, and alcohol consumption), body mass index (BMI), premorbid history, family history, and blood level measurement using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). None of the respondents from cases and control group have blood lead concentrations of more than 10μg/dL. Median and standard deviation of the blood lead level for case and control groups were 3.72 ± 5.46μg/dL and 2.81 ± 2.14μg/dL respectively. However, there was not a significant difference of the blood lead level between cases and control groups (Z = -1.48; p>0. 05). The relationship test showed that there was a significant relationship between blood lead concentration of 5 µg/dL and MI. (χ2 = 4.397, p = 0.03). In conclusion, this study has shown that there is a relationship between blood lead levels and MI. The blood lead level of cases is higher than non-cases, but still under the level of 10 μg/dL. This study concluded that the presence of lead in the blood is one of the factors that contribute to MI even at the lower concentration. The findings of this study are very important and provide new information regarding lead and myocardial infarction risk. 2019-08-23T00:00:00Z https://ptsldigital.ukm.my/jspui/handle/123456789/782849 Title: Pengesanan mikrosporidia usus dalam kalangan murid melayu sekolah rendah di daerah Besut, Terengganu menggunakan kaedah tindakbalas berantai polimerase SYBR green masa sebenar Authors: Rozliana Hanim Mat Hasan (P76981) Abstract: Data dan maklumat berkaitan prevalen mikrosporidiosis di kalangan individu yang sihat amat terhad di Malaysia. Ini adalah kerana kajian terhadap mikrosporidia seringkali difokuskan kepada pesakit yang terimunokompromi serta populasi Orang Asli. Tambahan lagi, kebanyakan kajian terdahulu menggunakan kaedah mikroskopi sahaja dalam metodologi pengesanan yang menyebabkan tidak banyak diketahui mengenai spesies mikrosporidia yang menjangkiti individu di dalam komuniti. Justeru, kajian ini telah dilakukan terhadap komuniti pelajar sekolah rendah Melayu yang sihat berusia lapan (8) hingga sebelas (11) tahun di dua buah sekolah di kawasan pedalaman daerah Besut, Terengganu bagi menentukan prevalen serta faktor risiko yang menyumbang kepada jangkitan mikrosporidia. Objektif lain kajian ini adalah untuk membandingkan spesifisiti dan sensitiviti saringan antara dua kaedah iaitu kaedah mikroskopi dengan kaedah tindakbalas rantaian polymerase masa sebenar (RT-PCR) berasaskan SYBR Green sebagai kaedah rujukan. Sebanyak 139 sampel tinja telah diproses dan diwarnakan dengan Gram Chromotrope Kinyoun terubahsuai. Dari jumlah ini, 134 sampel menjalani pemeriksaan mikroskopi dan 130 diuji dengan PCR masa sebenar. Pemeriksaan mikroskopi mendapati, sebanyak 41 (30.6%) sampel tinja adalah positif mikrosporidia berbanding 46 (35.4%) melalui kaedah PCR masa sebenar. Tiga belas (13) produk yang diamplifikasi telah dipilih menjalani pengesahan lanjut melalui penjujukan mendapati, 10 sampel adalah sepadan dengan gen piawai bagi mikrosporidia Sprague lophii small subunit ribosomal RNA gene, partial sequence dengan peratusan identifikasi 76% hingga 96%, 1 sampel sepadan dengan gen RNA 16S ribosomal RNA Kabatana takedai partial sequence manakala 2 lagi sampel sepadan dengan Kabatana sp. TM-2020 CM19 gene for 18S rRNA, partial sequence. Kaedah PCR masa sebenar SYBR Green bagi diagnosis mikrosporidia usus telah berjaya dioptimasi sebagai alternatif kepada kaedah mikroskopi dengan had pengesanan (LOD) 2.06 salinan/µl tindakbalas. Prevalen jangkitan pula nyata sekali lebih tinggi bagi subjek dengan pendapatan keluarga kurang darirpada RM1000 (OR=0.432, 95% CI=0.195,0.954 P=0.038). Pengesahan lanjut melalui analisa logistik regresi telah menunjukkan prevalen dalam kalangan subjek yang melakukan aktiviti mandi sungai/kolam (OR=0.035, 95% CI= 0.117,0.792 dengan nilai p<0.05) adalah faktor risiko yang nyata kepada jangkitan. Oleh itu, kajian ini menyimpulkan bahawa terdapat pendedahan terhadap jangkitan mikrosporidiosis dalam kalangan individu sihat. PCR masa sebenar pula boleh dijadikan sebagai salah satu kaedah alternatif bagi pengesanan mikrosporidiasis. Adalah dicadangkan agar kajian pada masa akan datang lebih fokus kepada individu atau komuniti yang immunokompeten. Ini adalah untuk menambah data dan membantu dalam program kawalan dan pencegahan jangkitan terutamanya kepada populasi immunokompromi yang lebih tinggi risiko untuk mendapat jangkitan. 2021-01-29T00:00:00Z